amigos podem namorar RNA-seq; normalize read count RPKM=(number of mapping reads)*1000bp*1 million reads/[(length of transcript)*(number of total reads )] http://www.clcbio.com/manual/genomics/Definition_RPKM.html http://seqanswers.com/forums/showthread.php?t=10657 http://genomics.isi.edu/rnaseq/framework http://www.nature.com/nmeth/journal/v5/n7/pdf/nmeth.1226.pdf