Vérifiez-le 10,000 hour challenge of microbe

Tag: IGV

  • Using gff format in IGV 1 Do not…

    brest gay Using gff format in IGV.

    1. Do not omit the first line, especially for GFF 3.
    As IGV supports both of GFF ver 2 and GFF ver 3, the file needs to be distinguished.
    Do not miss the first line

    ##gff-version    3

    2. Make sure tab is used to separate fields.
    Even for the first line, there must be a tab between gff-version and 3.
    No space.

    Here are the specifications.

    GFF version 2
    http://www.sanger.ac.uk/resources/software/gff/spec.html

    GFF version 3
    http://www.sequenceontology.org/gff3.shtml

  • Index fasta file generating fai file to use…

    Index fasta file – generating fai file – to use with IGV or bedtools.

    samtools faidx reference.fasta

    http://genomeview.org/content/preparing-fasta-files